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Stoichiometry and Monod-Andrews Growth Test Problems

Test problems on stoichiometry, growth yield, oxygen demand, and Monod-Andrews inhibited growth equations. Includes worked balances and growth formulas.

Category: Science

Uploaded by Melissa Grant on Apr 22, 2026

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Test problems 1-5

1. If -1 S = -6(X/4) O2 # Yx4 = 0?

need Y to write

stoichiometry for oxygen

Growth ONLY

Stoichiometry

true growth yield - can be measured exactly

R = Rd - feRa - fsRc

fs = YH (COD basis)

fc = 1 - YH

R = Rd - (1 - YH)Ra - YH Rc

2a. YH = 0.6 mgX-COD / mgS-COD = fs

MOLAR

Ss = C10H19O3N

Rd = 1/50 C10H19O3N + 9 H2O -> 9/50 O2 + 1/50 NH4+ + 1/50 HCO3- + 1/50 H+

- 0.6 Rc = 0.12 CO2 + 0.03 HCO3- + 0.03 NH4+ + 0.04 H2 + 0.6 -> 0.03 C5H7NO2 + 0.21 H2O

- 0.4 Ra = 0.1 O2 + 0.4 H+ + 0.4 e- -> 0.24 H2O

0.02 C10H19O3N + 0.01 NH4+ + 0.15 O2 + 0.01 HCO3- -> 0.11 H2O + 0.03 C5H7NO2 + 0.06 CO2

x50

C10H19O3N + 0.5 NH4+ + 5 O2 + 0.5 HCO3- -> 5.5 H2O + 1.5 C5H7NO2 + 3 O2

LHS RHS

C 10.5 10.5

N 1.5 1.5

O 17.5 17.5

H 21.5 21.5

charge 0 0

Σν reactant = Σν product

3. mw C10H19O3N = 201

ψ1 = 1, ψ2 = 0.5(18)/201 = 0.045, ψ3 = 5(32)/201 = 0.796

ψ4 = 0.5(61)/201 = 0.152, ψ5 = 5.8(18)/201 = 0.493, ψ6 = 1.5(13)/201 = 0.843

ψ7 = 3(44)/201 = 0.657

LHS: 1.993, RHS: 1.993

C10H19O3N + 0.015 NH4+ + 0.796 O2 + 0.152 HCO3- -> --

-> 0.493 H2O + 0.843 C5H7NO2 + 0.657 CO2

4. C10H19O3N : 1.99 gCOD / g OM

C10H19O3N-COD + 0.796(-1)/1.99 O2 -> 0.843(1.42)/1.99 C5H7NO2

C10H19O3N-COD + (-0.40) O2 -> 0.60 C5H7NO2

5. Monod: μ = μ̂ Ss / (Ks + Ss)

Andrews (inhibition) μ = μ̂ Ss / (Ks + Ss + Ss²/KI)

rg = μXB = rs / (1/YH) = ro / (-(1-YH)/YH) = ro / (-(1-YH)/YH)

μ̂

max. inhibited growth rate

μ* = μ̂ / (2(Ks/KI)^0.5 + 1)

S* = (KsKI)^0.5

μ̂

μ*z

μ*z

Monod and KI -> ∞

Andrews

KI1

KI2

Ks - same in both models but no longer gives 1/2 max rate in Andrews

K12 < K1

if KI = Ss

μ̂ = μ̂ KI / (Ks/KI + KI + KI) = μ̂ / (2 + Ks/KI) < μ̂/2 unless KI >> Ks

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